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Camptothecin (CPT) and topotecan (TPT) are DNA-targeting topoisomerase I (Top1) poisons. Top1 poisons stimulate DNA strand breaks, causing DNA damage and cell death. The aim of this work was to develop a robust, sensitive, selective and accurate high-performance liquid chromatography method for simultaneous analysis of CPT and its major metabolite (CPT-7-OH) and TPT and its major metabolite (TPT-OH) in plasma. Protein precipitation with acetonitrile was used to extract CPT and its metabolite from the plasma. Chromatographic separations were achieved on an ODS Hypersil C(18) column (250 x 4.6 mm, 5 microm) using acetonitrile-ammonium acetate buffer (80:20, v/v, pH 7.5; 20 mM) as the mobile phase. The flow rate was 1.5 mL/min, the column oven temperature was 40 degrees C and the injection volume was 10 microL. The detection wavelength was 230 nm. Linear calibration curves were obtained in the range of 0.1-50 microg/mL for CPT and TPT. The linear regression coefficient was >0.99. The detection limits were 0.05 and 0.08 microg/mL for CPT and TPT, respectively. The RSD was Organic semiconductor compositions are materials in which a large proportion of the molecules in the composition are in an organic phase, for example organic molecules such as a conjugated 0b46394aab