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The final test of the viability of living microorganisms in feed, food, or pharmaceutical products is the in vitro digestion simulation, also known as the simulated gastric fluid (SGF) test. This test’s purpose is to determine the stability of food or feed pellets during an in vivo digestion process. For this type of analysis, the sample to be tested is mixed with an artificial gastric fluid (test medium), and the mixture is incubated for a defined period of time. The result of the analysis is based on the change in mass of the sample.
The final test of the viability of living microorganisms in food or feed surfaces, such as raw fruits or vegetables, can be a chemical test or an in situ x-ray test. For the chemical test, the processing steps include: the sampling procedures (grab sample or band sample); the transport (in a bag or container); the sampling procedures (grab sample or band sample); the enrichment; the isolation; and the identification by both molecular and phenotypic methods. The U.S. Food and Drug Administration (FDA) recognizes three antimicrobial preservation methods as acceptable for food and feed surfaces: refrigeration; 4,50 freezing using ultra-high temperature (50) storage;51 and freezing using low temperature (50) storage.52 The procedure starts with sampling and transportation, then enclosure, and the prevention of contamination. The USDA also separates the three categories of risk in terms of pathogenic contamination: category I, no health risk; category II, low risk; and category III, high risk. 53 d2c66b5586